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A purified protein is in a Hepes N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid) buffer at pH 7 with 300 mM NaCl.
Posted: Mon Oct 04, 2021 8:07 am
by answerhappygod
- A Purified Protein Is In A Hepes N 2 Hydroxyethyl Piperazine N 2 Ethanesulfonic Acid Buffer At Ph 7 With 300 Mm Nacl 1 (45.64 KiB) Viewed 207 times
A purified protein is in a Hepes N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid) buffer at pH 7 with 300 mM NaCl. A dialysis membrane tube holds a 4.5 mL sample of the protein solution. The sample tube floats in a beaker containing 1.75 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Nat. C, and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. [NaCl) after a single dialysis: mM Calculate the final NaCl concentration in the 4.5 mL protein sample after dialysis in 325 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession (NaCl) after a double dialysis: mM