Answer Happy

What is the sequence of the bases in the mRNA coding for
the peptide above? What amino acid was altered in the normal
peptide and what amino acid replaced it to the mutant peptide?
(Question full and/or additional details on the attached
photo)
Failure of anti-oxidant function results in the hydroxylation of an aromatic acid of Enzyme Z and its activation, so that it degrades protoporphyrin to porphyrin, an unstable product. When hit by light, this product further degrades to form a compound responsible for the lesions and excruciating pain the man suffers. The mutation also affected an amino acid at the N-terminal of Enzyme X. Sequencing of the first seven (7) amino acids at the N-terminal of the normal enzyme gave the following sequence: trp-arg-asp-leu-ser-gly-his When the cDNA was sequenced by the Sanger method utilizing ddCTP, the following products were obtained: Tetranucleotide, Hexanucleotide, Nonanucleotide, Decanucleotide, Dodenucleotide, Octadecanucleotide, Nonadecanucleotide, 21-nucleotide What is the sequence of the bases in the mRNA coding for the peptide above? The mutation involved the 19 bases of the template strand of the peptide. A comparison of the electrophoretic profile of the normal peptided (N) and and mutant peptide (M) is shown below. The (+) electrode is situated at the bottom. What amino acid was altered in the normal peptide and what amino acid replaced it o the mutant peptide? pH 5.0 pH 7.0 pH 9.5 pH 10.5 DAGE - - NM NM NM NM