Answer Happy

Your colleague hands you a solution of purified protein in solution. She assures you that the solution does not contain any other impurities. However, none of the experiments she carried out using this protein have resulted in reproducible experiments. Confused, she asks you for help. She also explains that the protein is prone to forming agregates at higher concentrations. You subject a high concentration of the protein solution to a size exclusion column. The results are shown below Peak B Peak A Absorbance Л ti N 122*3************ »nnn***** Volume eluted (ml) However, when you repeat this experiment with low concentration of protein solution one of the peaks disappear You next run the protein solution through a ion exchange resin. No matter what concentration is run you always end up with the same result shown below. Absorbance ********************* Volume eluted (mL) 3) if the protein solution has no impurities, what is the expected result from running the ion exchange column? How many peaks do you expect to see? (2 points) 4) Do the results from the ion exchange column match your expected results? (1 point) 5) Provide a hypothesis explaining these observations. Do you think the protein solution is pure? Your hypothesis MUST be able to explain both sets of graphs shown above (3 points)