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1. Write the van Deemter equation and define or explain the terms in the equation. Sketch the van Deemter plot with labeled axes and describe how the terms or parameters in the equation affect the shape of the plot. Use the equation to explain why a 0.25 mm i.d. WCOT column has a higher resolution than a 1/8 in. packed column in GC analysis. 2. The metal tag from a HPLC eolumn has the following specifications: C18, 4.6 x 250 mm, 5 μm, 100 A (pore size). Explain what these specifications mean. How do these specifications affect the Explain the difference between "hard" and "soft" ionization in mass spectrometry. Describe the principles of operation for one technique each of "hard" ionization and "soft" ionization used in GC-MS. På 744 Describe the principles of operation and the analytical applications of one GC and one HPLC detectors. Include diagrams if necessary. (6 points) gird metal tagidentifies SP, length, internal diameter & film thicknes Molinary