Mass Spectrometry The written assignment consists of analysing the data generated from an in-house LC-MS experiment. The

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Mass Spectrometry The Written Assignment Consists Of Analysing The Data Generated From An In House Lc Ms Experiment The 1 (144.07 KiB) Viewed 37 times

Mass Spectrometry The written assignment consists of analysing the data generated from an in-house LC-MS experiment. The background to the experiment is provided below. The report has to be writ- ten in ACS analytical chemistry format and submitted as agreed. The ACS format/template is attached. The references should also be in ACS format and cited using Endnote X8 or higher. A basic tutorial is attached here. There are a number of videos available on YouTube that could be referred if a need arises. You can also get in touch with me in case of doubts. Background to the experiment for quantitative analysis We received samples from a company X to analyze and quantify lactic acid produced from different metabolic pathways in their bacterial cultures. The samples were diluted 10 folds in -80°C cold methanol and centrifuged at 14000 rpm for 15 minutes. The clear supernatant was filtered using 0.2 µM filter and transferred to LC-MS vials. An amount of 5 µL of sample was injected into single quadrupole LCMS instrument (Shimadzhu LCMS 2020). The samples were separated on a Waters Acquity UPLC BEH amide columns (2.1 mm X 150 mm, 100 Å pore size, 1.7 µm particle size) using an isocratic gradient of 35% B over 5 minutes. The mobile phase compositions are A (95 % MQ water, 5% acetonitrile and 0.1% formic acid) while mobile phase B (95 % acetonitrile, 5 % water and 0.1 % formic acid). The samples were run in both positive and negative ionization mode. A set of calibra- tion standards were prepared in water and also analyzed using the matched methods. After the analysis was completed, the data was converted into mzML format using MS convert (Proteowizard 2.0). The processed data for both positive and negative ionisation is attached below. Based on the data below prepare a report and discuss the results with appropriate references cited. The following things are attached below 1) Spectra of lactic acid in positive ionization mode. 2) Spectra of lactic acid in negative ionization mode. 3) Spectra of lactic acid from the bacterial samples in positive ionzation. 4) Spectra of lactic acid from the bacterial samples in negative ionzation. 5) Table listing peak areas for lactic acid in positive and negative ionisation at different concentrations. 1 *English decimal system followed