PLEASE ALSO SHOW HOW TO GET EQUATION You obtained the following raw data when setting up a Biuret standard curve: BSA (m
Posted: Wed May 04, 2022 4:01 pm
PLEASE ALSO SHOW HOW TO GET EQUATION
You obtained the following raw data when setting up a Biuret
standard curve:
BSA (mg/ml) Absorbancy 540nm
0 0.158
1 0.210
2 0.260
3 0.305
4 0.360
5 0.410
6 0.455
7 0.510
8 0.530
9 0.550
10 0.554
After blanking against a biuret-dH2O sample, the protein
concentration of an unknown sample was determined using the same
method and an absorbancy of 0.257 was obtained.
Set up a standard curve, excluding outliers (experimental and
statistical) and determine the protein concentration in the unknown
sample in mg / ml (up to 3 significant figures).
You obtained the following raw data when setting up a Biuret
standard curve:
BSA (mg/ml) Absorbancy 540nm
0 0.158
1 0.210
2 0.260
3 0.305
4 0.360
5 0.410
6 0.455
7 0.510
8 0.530
9 0.550
10 0.554
After blanking against a biuret-dH2O sample, the protein
concentration of an unknown sample was determined using the same
method and an absorbancy of 0.257 was obtained.
Set up a standard curve, excluding outliers (experimental and
statistical) and determine the protein concentration in the unknown
sample in mg / ml (up to 3 significant figures).