The resolution factor R for two solutes 1 and 2, whose elution peaks are adjacent, is sometimes expressed by equation (1
Posted: Wed May 04, 2022 3:40 pm
The resolution factor R for two solutes 1 and 2, whose elution peaks are adjacent, is sometimes expressed by equation (1): fR(2)-R(1) R= (1) W1/2(1) + 1/2(2) 1 -α-1 k₂ R= (2) 4 a 1+k₂ a. Show that this relation is different from the basic one by finding the expression corresponding to the classic equation which uses wb, the peak width at the baseline. If it is revealed that the two adjacent peaks have the same width at the baseline (w1 =w2), then show that relation (2) is equivalent to relation (1) for the resolution. b.