Show the preparation of 500ml, 10X Tris-HCl buffer, pH=9.5, and then use it to prepare 40ml of 100mM Tris-HCl buffer, pH
Posted: Fri Apr 29, 2022 12:43 pm
Show the preparation of 500ml, 10X Tris-HCl buffer, pH=9.5, and then use it to prepare 40ml of 100mM Tris-HCl buffer, pH=9.5, containing 0.25M NaCl and 5MM GSH. (Tris mw=121g/mole, NaCl mw=58.5g/mole, GSH mw=307.3 g/mole)