- Age 1 N 24 3 Gfp Is Approximately Kd 30 B 42 27 63 Submit Quiz 2 Of 3 Questions Saved Match The Steps Of Pro 1 (35.47 KiB) Viewed 29 times
- Age 1 N 24 3 Gfp Is Approximately Kd 30 B 42 27 63 Submit Quiz 2 Of 3 Questions Saved Match The Steps Of Pro 2 (29.12 KiB) Viewed 29 times
Match the steps of protein extraction with their specific purpose. 2- 5- 1 3 4 Lysozyme solution Resuspend the pellet in TE buffer TE buffer to hydrophobic column Binding buffer Wash Buffer 1. Contains EDTA which is a chelating agent binding to the positive charges in the suspension 2. Contains an enzyme to breakdown the cell wall and release intracellular GFP 3. Changes the charge of the protein to allow the GFP with bind to the column 4. Aids in removing any proteins that have different binding properties than GFP 5. Elutes the GFP from the column