(20pts) Analysis of Analgesics by Thin Layer Chromatography From the procedure 1. Obtain a 400-ml beaker to use as the d

[answerhappygod]

20pts Analysis Of Analgesics By Thin Layer Chromatography From The Procedure 1 Obtain A 400 Ml Beaker To Use As The D 1 (44.04 KiB) Viewed 39 times

20pts Analysis Of Analgesics By Thin Layer Chromatography From The Procedure 1 Obtain A 400 Ml Beaker To Use As The D 2 (18.36 KiB) Viewed 39 times

20pts Analysis Of Analgesics By Thin Layer Chromatography From The Procedure 1 Obtain A 400 Ml Beaker To Use As The D 3 (30.34 KiB) Viewed 39 times

20pts Analysis Of Analgesics By Thin Layer Chromatography From The Procedure 1 Obtain A 400 Ml Beaker To Use As The D 4 (14.49 KiB) Viewed 39 times

20pts Analysis Of Analgesics By Thin Layer Chromatography From The Procedure 1 Obtain A 400 Ml Beaker To Use As The D 5 (18.36 KiB) Viewed 39 times

20pts Analysis Of Analgesics By Thin Layer Chromatography From The Procedure 1 Obtain A 400 Ml Beaker To Use As The D 6 (15.51 KiB) Viewed 39 times

(20pts) Analysis of Analgesics by Thin Layer Chromatography From the procedure 1. Obtain a 400-ml beaker to use as the developing chamber, enough plastic wrap to cover the mouth of the beaker, and a rubber band. 2. Pour a small amount of solvent into the chamber so it is -0.55 cm deep. The solvent should be below the spots on the plate. 3. Cover the beaker with the plastic wrap, securing it with a rubber band. 4. Obtain a TLC plate. Avoid touching the plate on the surface. Only handle it at the edges. 5. Mark the origin, as shown in Figure A.5, by drawing a line in pencil-1 cm from the bottom of the plate. 6. On the origin, mark 6 equally spaced dots and number them 1 through 6. 1. Aspirin 2. Ibuprofen 3. Acetominophen 4. Naproxen 5. Caffeine 6. Unknown over the counter medication 7. Using a separate and clean capillary pipette for each solution, spot a very small amount of each solution onto the dots on the TLC plate. 8. Remove the plastic wrap from the chamber and carefully place the TLC plate into the developing chamber ensuring that it enters the solvent evenly and that the solvent is below the origin. 9. Cover the chamber with the plastic wrap and secure it with the rubber band. 10. Allow the solvent to travel up the plate undisturbed. 11. Once the solvent is=1 cm from the top of the plate, remove the plastic wrap and remove the plate. 12. Mark the solvent front on the plate with a pencil and allow the plate to dry in the hood 13. Dispose of materials as instructed by your TA or lab instructor
Virtual TLC Analysis The image shown below represents a TLC was prepared according to the procedure above. The lanes on the TLC are labelled as follows: 1. Aspirin 2. Ibuprofen 3. Acetominophen 4. Naproxen 5. Caffeine 6. Unknown over the counter medication After developing the TLC plate, the solvent front was marked with a pencil and it was placed under a UV light. The background of the plate fluoresces green with compounds that absorb UV-light appearing as darker spots. The spots are circled with a pencil. Use the following image to 'measure the distance traveled by the solvent and by each of the spots
1 2 3 4 5 -5 -3 Complete the following table with your measurements and calculate the Ry of each spot. Report distances to at least the nearest 0.1 cm. Note: Your unknown sample may contain one, two, or three analgesics.
. If your sample only has one spot, enter it in the row for the "lower or only spot" • If your sample has two or more spots, the lower spot (that did not travel as far) is listed first and the upper spot (which traveled the furthest) is listed last. If a there are more entries than needed for your unknown sample, enter zero for the excess distance traveled and R, entries. Do NOT leave them blank. (1pts) Enter the distance the solvent travelled (cm)
Lane #1: Aspirin Lane #2: Ibuprofen Lane #3: Acetaminophen Lane #4: Naproxen Lane #5: Caffeine Lane #6: Unknown 4 Lower or only spot smallest distance traveled if multiple Middle spot (enter zero if none present) Upper spot (enter zero if none present) largest distance traveled if multiple Distance spot traveled (cm) R₁
Based upon the TLC analysis, what is the likely most identity of each spot in Unknown 4? (2pts) Lower or only spot (smallest distance traveled if multiple) (2pts) Middle spot (2pts) Upper spot (largest distance traveled if multiple) (1pts) Which brand of OTC pain reliever best matches the composition of unknown 47 Choose Choose... Choose... Choose...