1. a) Briefly describe how recombinant DNA technology can be used to modify a wild strain of Escherichia coll into an in

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1. a) Briefly describe how recombinant DNA technology can be used to modify a wild strain
of Escherichia coll into an insulin-producing strain
(4 marks)
b) Briefly describe three techniques that would be employed in the above procedure.
(6 marks)
c) List two methods for purifying the DNA from E coll in the above procedure (la).
d) Briefly discuss, with an example, how Escherichia coli protects itself against the T4phage.
d) Briefly discuss, with an example, how Escherichia coli protects itself against the T4
phage.