Forensic Genetics Assignment: An alleged brawl occurs outside a bar and a sample, that appears to be a mixture of blood

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Forensic Genetics Assignment:
An alleged brawl occurs outside a bar and a sample, that appears to be a mixture of blood and mud, was collected. The sample was collected at the scene by an investigator and it was submitted to the laboratory for DNA analysis.
List five important factors that may compromise a final accurate conclusion of the analysis. Explain the possible effect of the factors you have chosen on the analysis or conclusion, indicating where, in the process from sample collection to production of the report, these might have an influence.