Chromatography Problem Solving 1 (387.94 KiB) Viewed 88 times
Chromatography Problem Solving 2 (518.85 KiB) Viewed 88 times
Problem Solving (Chromatography) In a paper chromatography, experiment a student runs a sample of a mixture of amino acids with standard samples of glycine, alanine and phenylalanine. solvent front spots visible after running chromatograph and development 120 cm chromatography baper 12 cm 9 cm 4 cm start line Glycine Phenylalanine Alanine X Sample solvent a. (selecting) Using the image of the chromatography paper above, state one amino acid that is present in the sample. b. (analysing) The student me red the migration distance of the solvent front as 20 cm. The migration distances of the amino acid standards were recorded in the table. The student calculated the retention factor (Rf), as a measure of the degree to which the substance is retained by the stationary phase, for samples using the following formula: R; migration distance of substance migration distance of solvent front Substance Molecular Mass Rf Migration distance of substance (cm) (g/mol) Glycine 89.1 12 0.6 Alanine 75.1 9 0.45 Phenylalanine 165.2 4.
write a statement that describes the general trend between the migration distance of the substance and molecular mass. c. (processing) Calculate the Rf of Phenylalanine. d. (predicting) Leucine has a molecular mass of 131.2 g/mol. Predict the migration distance of Leucine on the chromatography paper. (It is acceptable to: indicate this on a diagram, or describe the migration distance in relation to the migration of other substances in table, or state a value in cm for the migration distance)
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