Question 4 Depending on your knowledge about the quantitative applications of UV spectroscopy (Beer's Law), answer the f

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Question 4 Depending On Your Knowledge About The Quantitative Applications Of Uv Spectroscopy Beer S Law Answer The F 1 (47.43 KiB) Viewed 84 times

Question 4 Depending on your knowledge about the quantitative applications of UV spectroscopy (Beer's Law), answer the following questions 1. The transmittance for a sample of a solution, measured at 590 nm in a 1.5 cm cuvette, was 50.2%. a) What is the corresponding absorbance? b) If the concentration is 0.006 M, what is the molar absorptivity of the analyte at this wavelength? C) If the absorbance obtained in question (a) increased by a factor of 3, what is the new transmittance of the solution? 2. Five standard solutions of X were prepared accurately and the absorbance of each standard was measured using UV-spectrometer. A calibration curve was build up and the curve was linear. The linear equation calculated from this curve was y=230x + 25. a) How we can measure the molar absorptivity of this solution from the calibration curve? b) State the effect on the calibration curve when 1. Polychromatic radiation is used as a source of radiation 2. The analyte dissociate in the solution 3. Higher concentrations of analyte were used 3. Give three reasons why determining max is important in UV-Visible spectroscopy?