Assume an E. coli strain is grown under conditions where there are both high levels of lactose and high levels of glucos

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Assume An E Coli Strain Is Grown Under Conditions Where There Are Both High Levels Of Lactose And High Levels Of Glucos 1 (56.91 KiB) Viewed 25 times

Assume an E. coli strain is grown under conditions where there are both high levels of lactose and high levels of glucose present. Give a detailed description of how this will impact the regulation of the lac operon, and what the result will be on the expression of the structural genes. 5. Give all possible genotypes of a lac operon that produces, or fails to produce, B-galactosidase and permease under the following conditions, following the example as completed for (a). Do not give partial diploid genotypes. Lactose absent Lactose present B-Galactosidase Permease B-Galactosidase Genotype: Permease + a. + laci lacP laco lacz lacY b. - + 3 C. - H d. + + + e. - 1 6. Consider the two E.coli strains with partial diploid genotypes with respect to the lac operon given below. In each case, predict whether the strains will synthesize B-galactosidase and permease in the absence and presence respectively; motivate your answer. a. lacl lacP laco lacZ lacy / lacl lacP lacO* lacZlacy b. laci lacP laco lacZ* lacY* / lac I* lacP laco lacz* lacy 4 - + + M