The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are

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The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are

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The Initial Rate For An Enzyme Catalyzed Reaction Has Been Determined At A Number Of Substrate Concentrations Data Are 1
The Initial Rate For An Enzyme Catalyzed Reaction Has Been Determined At A Number Of Substrate Concentrations Data Are 1 (40.82 KiB) Viewed 62 times
The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows: [S] (umol/L) 5 10 20 50 100 200 v (umol/L) min) 22 39 65 102 120 135 (a) Estimate Vmax and Km from a direct graph of v versus (S). Do you find difficulties in getting clear answers? (b) Now use a Lineweaver-Burk plot to analyze the same data. Does this work better? (c) Finally, try an Eadie-Hofstee plot of the same data. (d) If the total enzyme concentration was 1 nmol/L, how many molecules of substrate can a molecule of enzyme process in each minute? (e) Calculate kaat/Km for the enzyme reaction. Is this a fairly efficient enzyme?
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