COURSE & CODE: BIOC201 - Introduction to Biomolecules Page 17 1. (a) Describe the preparation of 500 ml of a 200 mM sodi

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Course Code Bioc201 Introduction To Biomolecules Page 17 1 A Describe The Preparation Of 500 Ml Of A 200 Mm Sodi 1 (65.07 KiB) Viewed 31 times

COURSE & CODE: BIOC201 - Introduction to Biomolecules Page 17 1. (a) Describe the preparation of 500 ml of a 200 mM sodium acetate buffer, pH 4.5, containing 400 mM NaCl and 0.02% (w/v) NaN3. Show all calculations. [M,-values (g/mol):CH,COOH = 60; CH3COONa.3 H2O = 136.05; NaN, = 65.1; NaCl = 58.44). Density of 100% (v/v) CH3COOH = 1.05 g/ml [6] (b) Explain how you would prepare 250 ml of a 0.1 M HCI solution. The following information is given on the bottle of HCl: M, = 36.2; 34% (v/v); density = 1.16 g/ml. [4]